Start with your samples
Tell us your sample type, the biological question, the depth you need and what the final dataset has to support. We will define the appropriate preparation and sequencing strategy and quote it in writing within one working day.
If a workflow you need is not one we run routinely, we will say so. Some can be established for a confirmed project; others are better placed elsewhere, and we will point you there.
WHY library preparation & sequencing
Choose the sequencing approach that fits the biology
Whole genome sequencing
Deep WGS
Research-use variant detection at 30× coverage or above. Best suited to smaller genomes or modest sample numbers. For large human WGS cohorts, higher-throughput platforms will usually be more cost-effective, and we will tell you so.
Low-pass WGS
Approximately 0.1 to 5×, suited to structural variant, aneuploidy and copy number variant screening.
Feasibility and trial projects
Smaller genomes and feasibility studies scoped to project requirements.
Whole exome sequencing
Focused sequencing of protein-coding regions at high depth for variant discovery.
Coverage: 50 to 200×, customisable to your variant detection goals
Exome capture: leading enrichment kits covering approximately 60 Mb of exonic sequence
Primary applications: rare disease research and germline variant discovery
Standard output: demultiplexed FASTQ
RNA sequencing
Whole transcriptome
Unbiased profiling of expressed transcripts.
3’ quantification
Cost-effective measurement of transcript abundance.
Typical coverage
10 to 100 million paired-end reads per sample, customisable to the project.
RNA depletion
Ribosomal RNA depletion available for intact and degraded RNA, using species-specific kits including human, mouse and rat.
Depth, coverage and read length are set by what you need to detect, in what material and across how many samples – and agreed with you at scoping before anything is committed.
Input is assessed on the material you actually have, and we’ll confirm what’s workable before you ship.
WHY sequencing
What you gain
End-to-end workflow.
Library preparation and sequencing in one service, in one laboratory
Multiple sequencing strategies.
WGS, WES and RNA-seq
Depth set by the study
not by a service tier, and agreed with you before the run
2 to 3 weeks from sample receipt
where we prepare the libraries, with the date confirmed at quote
Senior scientific oversight
from sample preparation through sequencing
Element AVITI platform
for supported workflows
Intact and degraded RNA
both supported
FASTQ delivery as standard
with QC reporting alongside
Specialist workflows
can be established for confirmed projects
Sequencing alongside proteomics.
Where a programme needs both, they come from the same samples and the same laboratory
what and how
What you receive, and how analysis works
Delivered as standard
How genomics analysis works here
Proteomics-linked and multi-omic analysis is different
Bioinformatics and data analysis
why work with sequencing
Specialist workflows for confirmed projects
Some approaches are not part of the routine in-house portfolio but can be established for confirmed projects. We are explicit about which is which.
Quality control from preparation to delivery
Have samples ready for sequencing?
Tell us what you have, what you need to measure and what you need from the final dataset. We will define the appropriate library preparation and sequencing approach and quote it in writing.